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A sensitive one-step immunocapture EIA for rapid diagnosis of influenza A

Identifieur interne : 001C41 ( Main/Exploration ); précédent : 001C40; suivant : 001C42

A sensitive one-step immunocapture EIA for rapid diagnosis of influenza A

Auteurs : M. Tkác Ová [Slovaquie] ; E. Vare Ková

Source :

Journal of Virological Methods [ 0166-0934 ] ; 1996.

RBID : ISTEX:AB7EDBF6A13CD44B975E988A375E3E0F8F32B8D0

English descriptors

Teeft :
- Absolute sensitivity, Acta virol, Ammonium sulphate, Antigen dilution buffer, Antiviral drugs, Assay, Clinical sample buffer, Clinical specimens, Diagnostic test, Diagnostic unit, Enzyme immunoassay, Enzyme peroxidase, Epidemiological surveillance, Human influenza, Immunocapture, Immunocapture assay, Influenza, Influenza infection, Influenza type, Influenza virus, Influenza virus strains, Mabs, Microtitration plate, Molar ratio, Monoclonal, Monoclonal antibodies, Nasal washes, Nasopharyngeal washes, Nucleoprotein, Peroxidase, Rapid diagnosis, Rapid influenza, Reference antigen, Room temperature, Sensitive immunocapture, Solid phase, Standard antigen, Standard deviations, Viral, Viral antigen, Viral particles, Virol, Virological methods, Virus, Virus antigen, Virus culture confirmation assay, Virus strains, William dunn school.

Abstract

Abstract: A highly sensitive one-step immunocapture EIA for the detection of influenza A virus antigen directly in a clinical specimen was developed. The sensitivity was achieved by using two high-affinity cross-reactive influenza type A-specific monoclonal antibodies, recognizing independent nonoverlapping epitopes on the influenza A nucleoprotein. One of the two MAbs was used as a capture antibody, while the other was coupled with enzyme peroxidase and served as a detector. Sensitivity to detection of highly purified recombinant influenza A virus nucleoprotein by EIA reached approximately 10 pg. Fifteen purified human influenza A virus strains of H1, H2 and H3 subtypes, isolated during the period 1934–1992, were tested by this system. All the influenza A viruses tested positive, whereas two influenza B viruses used as a control were negative. The efficiency of the system for detection of influenza A viral antigen directly in clinical specimens was confirmed by testing nasal and nasopharyngeal washes and aspirates, tested previously by time-resolved fluoroimmunoassay and by virus culture confirmation assay.

Url:

https://api.istex.fr/ark:/67375/6H6-FH9WS1JB-7/fulltext.pdf

DOI: 10.1016/0166-0934(96)02046-0

Affiliations:

Slovaquie

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Le document en format XML

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<front><div type="abstract" xml:lang="en">Abstract: A highly sensitive one-step immunocapture EIA for the detection of influenza A virus antigen directly in a clinical specimen was developed. The sensitivity was achieved by using two high-affinity cross-reactive influenza type A-specific monoclonal antibodies, recognizing independent nonoverlapping epitopes on the influenza A nucleoprotein. One of the two MAbs was used as a capture antibody, while the other was coupled with enzyme peroxidase and served as a detector. Sensitivity to detection of highly purified recombinant influenza A virus nucleoprotein by EIA reached approximately 10 pg. Fifteen purified human influenza A virus strains of H1, H2 and H3 subtypes, isolated during the period 1934–1992, were tested by this system. All the influenza A viruses tested positive, whereas two influenza B viruses used as a control were negative. The efficiency of the system for detection of influenza A viral antigen directly in clinical specimens was confirmed by testing nasal and nasopharyngeal washes and aspirates, tested previously by time-resolved fluoroimmunoassay and by virus culture confirmation assay.</div>
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A sensitive one-step immunocapture EIA for rapid diagnosis of influenza A

A sensitive one-step immunocapture EIA for rapid diagnosis of influenza A

Source :

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri